Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BRÖKELMANN, J.
Right arrow Articles by FAWCETT, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BRÖKELMANN, J.
Right arrow Articles by FAWCETT, D. W.
Agricola
Right arrow Articles by BRÖKELMANN, J.
Right arrow Articles by FAWCETT, D. W.

Biology of Reproduction, Vol 1, 59-71, Copyright © 1969 by Society for the Study of Reproduction

The Localization of Endogenous Peroxidase in the Rat Uterus and Its Induction by Estradiol

JOST BRÖKELMANN 1, and DON W. FAWCETT 1

1 Department of Anatomy and the Laboratories of Human Reproduction and Reproductive Biology, Harvard Medical School, Boston, Massachusetts 02115


Application of an electron-microscopic histochemical staining method to rat uterus demonstrated endogenous peroxidase activity in erythrocytes, blood and tissue eosinophils and in the uterine epithelium. The reaction in the epithelium was localized in the endoplasmic reticulum and nuclear envelope. Under certain conditions a positive reaction, possibly attributable to cytochrome oxidase was also present in the intracristal space and between the limiting membranes of the mitochondria.

The epithelial "peroxidase" was sensitive to cyanide and carbon monoxide. It was normally present only during estrus but could be induced in intact or ovariectomized rats by administration of estradiol. The first signs of peroxidase activity appeared 12 hr after estrogen treatment in segments of the endoplasmic reticulum scattered through the cytoplasm. The activity subsequently spread throughout the reticulum and into the nuclear envelope but the Golgi complex and nucleoplasm remained negative.

The induction of "peroxidase" in the estrogen stimulated uterus could be prevented by administration of an inhibitor of protein synthesis. It is believed that this epithelial "peroxidase" or hemoprotein may be involved in the metabolism of estradiol in the uterus.

 Submitted on November 15, 1968




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
R. D. Blumenthal, M. Samoszuk, A. P. Taylor, G. Brown, R. Alisauskas, and D. M. Goldenberg
Degranulating Eosinophils in Human Endometriosis
Am. J. Pathol., May 1, 2000; 156(5): 1581 - 1588.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1969 by the Society for the Study of Reproduction.