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Biology of Reproduction, Vol 10, 47-53, Copyright © 1974 by Society for the Study of Reproduction

Intercellular Transport of Steroids in the Infused Rabbit Testis

L. G. VAN DOORN 1, H. W. A. DE BRUIJN 1, H. GALJAARD 1, , and H. J. VAN DER MOLEN 1

1 Department of Cell Biology and Genetics, Department of Biochemistry, Division of Chemical Endocrinology, Medical Faculty at Rotterdam, Rotterdam, The Netherlands


[3H]Pregnenolone2 and [3H]testosterone were infused via the testicular artery into the rabbit testis in situ, in order to determine if steroids can pass the "blood-testis barrier." After various periods of infusion (5-60 min) the testes were frozen, cryostat sections were cut and freeze-dried. Interstitium and tubules were isolated by micro dissection and radioactivity per µg dry weight was measured in both tissue compartments. Radioactive steroids could be isolated from the interstitial tissue as well as from the seminiferous tubules. After infusion of [3H]pregnenolone in blood, the ratio (dpm 3H/µg tubules):(dpm 3H/µg interstitium) varied from 0.46 to 0.97. When Krebs-Ringer bicarbonate buffer was used as a perfusion medium, the same degree of penetration was observed, but after longer periods of infusion (60 min). Separation and identification of the radioactive steroids after infusing [3H]pregnenolone showed that pregnenolone and testosterone were the major radioactive steroids in both interstitium and seminiferous tubules.

After infusion with [3H]testosterone, both [3H]testosterone (77%) and [3H]androstenedione (23%) were found in the seminiferous tubules.

It is concluded that steroids can be transported from the Leydig cells to the seminiferous tubules.

 Accepted on May 30, 1973






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Copyright © 1974 by the Society for the Study of Reproduction.