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Biology of Reproduction, Vol 10, 62-68, Copyright © 1974 by Society for the Study of Reproduction

Fertilization Studies with Egg Sections of the Horseshoe Crab, Limulus polyphemus L

The Effects of Bivalent and Univalent Anti-egg Antibodies on Sperm—Egg Attachment

RODNEY C. MOWBRAY 1, and GEORGE GORDON BROWN 1

1 Department of Zoology and Entomology, Iowa State University, Ames, Iowa 50010


Following insemination in the horseshoe crab (Limulus polyphemus), large numbers of spermatozoa normally attach to the egg surface. Such polyspermic attachment will even occur after freezing and thawing of Limulus eggs. Finally, this attachment also results when thin frozen sections of eggs are inseminated. Such sections provide a simple, two-dimensional system for quantitative study of sperm—egg attachment. The effects of several variables on sperm—egg attachment were examined with this two dimensional system; these include duration of exposure to sperm, temperature, concentration of sperm, and the effects of antibodies.

Pretreatment of eggs with anti-egg or anti-egg envelope antibody preparations greatly reduced sperm—egg attachment, suggesting that attachment involves an egg antigen(s). This conclusion was further substantiated by demonstration that univalent (Fab) antibody fragments also inhibited sperm attachment.

 Accepted on June 5, 1973






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Copyright © 1974 by the Society for the Study of Reproduction.