Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MENGE, A. C.
Right arrow Articles by LIEBERMAN, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MENGE, A. C.
Right arrow Articles by LIEBERMAN, M. E.
Agricola
Right arrow Articles by MENGE, A. C.
Right arrow Articles by LIEBERMAN, M. E.

Biology of Reproduction, Vol 10, 422-428, Copyright © 1974 by Society for the Study of Reproduction

Antifertility Effects of Immunoglobulins from Uterine Fluids of Semen-Immunized Rabbits

A. C. MENGE 1, and M. E. LIEBERMAN 1

1 Center for Research in Reproductive Biology, Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan 48104


Fluids were collected from the ligated and stimulated uterine horns of rabbits that had been isoimmunized with semen and adjuvant both transvaginally and systemically or only transvaginally. Treating rabbit sperm with immune uterine fluids (IUF) decreased fertility in inseminated rabbits. The antifertility effect of IUF was attributed to antibody activity as it was removed by absorption with sperm and goat antisera against rabbit gamma globulins. The predominant immunoglobulins, IgC and secretory IgA, were separated from IUF by gel filtration Sephadex G-200 and by anion exchange on DEAE cellulose. The separated immunoglobulins were also treated with monospecific antisera against both Ig classes to remove possible cross contamination. The results indicated that both IgG and secretory IgA in IUF possessed specific sperm antibodies capable of depressing fertility in inseminated rabbits.

Accepted on October 26, 1973






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the Society for the Study of Reproduction.