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Biology of Reproduction, Vol 10, 502-511, Copyright © 1974 by Society for the Study of Reproduction

Development of the Mouse Blastocyst following Injection with Newcastle Disease Virus

ROBERT H. GLASS 1, PATRICIA G. CALARCO 1, T. P. LIN 1, JOAN FLORENCE 1, , and JANG O. OH 1

1 Departments of Anatomy and Pediatrics and Francis I. Proctor Foundation, University of California, San Francisco, California 94122


Newcastle disease virus was injected into the mouse blastocoele. Eleven or 272 (4.0%) injected eggs transferred to recipient mice survived to Day 17 of pregnancy. In contrast, 25.8% of blastocysts injected with allantoic fluid or phosphate-buffered saline before transfer were fully developed at Day 17.

When virus-injected blastocysts were cultured for 48 h, 47 of 66 degenerated, while 14 of 15 control-injected blastocysts appeared normal.

Electron microscopy of virus-injected blastocysts cultured for 24 h showed viral budding from the surface of the trophoblast cells facing the zona pellucida. In addition, the cells contained filamentous aggregates thought to be nucleocapsid. After 48 h in culture, the trophoblast cells also contained electron-dense granules. There was some degeneration of trophoblast and large numbers of pleomorphic viral forms were present. The inner cell mass did not show evidence of viral infection.

 Accepted on November 2, 1973






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Copyright © 1974 by the Society for the Study of Reproduction.