Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by INOUE, M.
Right arrow Articles by WOLF, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by INOUE, M.
Right arrow Articles by WOLF, D. P.
Agricola
Right arrow Articles by INOUE, M.
Right arrow Articles by WOLF, D. P.

Biology of Reproduction, Vol 10, 512-518, Copyright © 1974 by Society for the Study of Reproduction

Solubility Properties of the Murine Zona Pellucida

MASATO INOUE 1, and DON P. WOLF 1

1 Division of Reproductive Biology, Department of Obstetrics and Gynecology, School of Medicine, and Department of Biophysics, University of Pennsylvania Philadelphia, Pennsylvania


The solubility properties of the zona pellucida surrounding the intact unfertilized murine egg have been investigated. The structural integrity of the zona was shown to be disrupted by several disulfide-bond reducing agents. A correlation was established between the rate of zonae dissolution in mercaptoethanol and the concentration of the sulfhydryl anion. It was possible to conclude, therefore, that disulfide bonds play a role in maintaining the structural integrity of the murine zona pellucida. The effect of pH on zonae solubility was reinvestigated. Removal of cumulus cells with unbuffered solutions of hyaluronidase or exposure of ova to low pH led to dramatic alterations in the physicochemical properties of the resultant zonae. This study provides the basis for evaluating possible relationships between the occurrence of a zona reaction and changes in the physicochemical properties of the zona pellucida.

Accepted on November 2, 1973




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
J.M. Bedford
Mammalian Fertilization Misread? Sperm Penetration of the Eutherian Zona Pellucida Is Unlikely to be a Lytic Event
Biol Reprod, December 1, 1998; 59(6): 1275 - 1287.
[Full Text]

Home page
 Cold Spring Harb Symp Quant BiolHome page
P.M. Wassarman, J.D. Bleil, H.M. Florman, J.M. Greve, R.J. Roller, G.S. Salzmann, and F.G. Samuels
The Mouse Egg's Receptor for Sperm: What Is It and How Does It Work?
Cold Spring Harb Symp Quant Biol, January 1, 1985; 50(0): 11 - 19.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the Society for the Study of Reproduction.