Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WASSERMAN, W. J.
Right arrow Articles by MASUI, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WASSERMAN, W. J.
Right arrow Articles by MASUI, Y.
Agricola
Right arrow Articles by WASSERMAN, W. J.
Right arrow Articles by MASUI, Y.

Biology of Reproduction, Vol 11, 133-144, Copyright © 1974 by Society for the Study of Reproduction

A Study on Gonadotropin Action in the Induction of Oocyte Maturation in Xenopus laevis

WILLAM J. WASSERMAN 1, and YOSHIO MASUI 1

1 Department of Zoology, University of Toronto, Toronto 5, Ontario, Canada


The necessity of RNA synthesis within the ovarian follicle cells for gonadotropin-induced oocyte maturation was examined using isolated ovarian tissues of Xenopus laevis. Studies using [5-3H]uridine revealed that the radioactivity incorporated into the RNA of the theca epithelium was much higher than that incorporated into the RNA of the follicle cells and oocyte. No difference was observed in the quantity of radioactivity that was incorporated into the RNA of hormone-treated ovarian tissues as compared to that for untreated tissues. However, inhibitor studies indicated that actinomycin D and agr-amanitin had a variable inhibitory effect on gonadotropin-induced maturation when they were applied to the ovarian follicles prior to the gonadotropin. On the contrary, no significant inhibitory effect on maturation was observed if these inhibitors were applied either simultaneously with or after the initiation of gonadotropin treatment, even though bulk RNA synthesis was always inhibited by approximately 85% with actinomycin D. Ethidium bromide, on the other hand, was always effective in inhibiting gonadotropin-induced maturation, although there was no detectable suppression in the incorporation of [3H]uridine into bulk RNA. All three drugs had no inhibitory effect on progesterone-induced maturation. A working hypothesis has been proposed that can account for the variable inhibitory effect of actinomycin D and agr-amanitin on gonadotropin-induced oocyte maturation.

Accepted on January 2, 1974




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
G. Maton, T. Lorca, J.-A. Girault, R. Ozon, and C. Jessus
Differential regulation of Cdc2 and Aurora-A in Xenopus oocytes: a crucial role of phosphatase 2A
J. Cell Sci., June 1, 2005; 118(11): 2485 - 2494.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. E. Ferrell Jr. and E. M. Machleder
The Biochemical Basis of an All-or-None Cell Fate Switch in Xenopus Oocytes
Science, May 8, 1998; 280(5365): 895 - 898.
[Abstract] [Full Text]

Home page
 ScienceHome page
W. Wasserman and Y Masui
A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization
Science, March 26, 1976; 191(4233): 1266 - 1268.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Tian, S. Kim, E. Heilig, and J. V. Ruderman
Identification of XPR-1, a progesterone receptor required for Xenopus oocyte activation
PNAS, December 19, 2000; 97(26): 14358 - 14363.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the Society for the Study of Reproduction.