Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 11, 162-167, Copyright © 1974 by Society for the Study of Reproduction

Development of Mammalian Embryos in Vitro with Increased Atmospheric Pressure

D. S. ELLIOTT 1, R. R. MAURER 1, , and R. E. STAPLES 1

1 Environmental Toxicology Branch Section on Reproduction and Ontogenesis, National Institute of Environmental Health Sciences (National Institutes of Health), Research Triangle Park, North Carolina 27709


Embryo sensitivity to pressure was determined by culturing preimplantation embryos of the rabbit, rat and mouse at atmospheric pressures of 1-64 in. H2O above ambient. The proportion of embryos that attained the blastocyst stage during culture was the criterion used to assess pressure sensitivity. A pressure of 64 in. H2O was detrimental to 2- to 4-cell rabbit embryos. However, lower pressures enhanced development of rabbit and rat embryos as evidenced by the significant quadratic response found. The development of 2- and 8-cell mouse embryos was less affected by increased pressures up to 64 in. H2O. At this pressure, significantly fewer 8-cell mouse embryos developed into blastocysts. Pressures of 16 in. H2O for the 2- to 4-cell rabbit embryo and 4 in. for 8-cell rat and 2-cell mouse embryos appear to enhance development above that obtained at low pressures.

Accepted on March 5, 1974






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Copyright © 1974 by the Society for the Study of Reproduction.