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Biology of Reproduction, Vol 11, 198-204, Copyright © 1974 by Society for the Study of Reproduction
1 Dairy Breeding Research Center, The Pennsylvania State University,
University Park, Pennsylvania 16802 Postpuberal changes in the distribution of neutral lipid and choline-containing phospholipid (CPL) in the epididymal epithelium were evaluated histochemically. Groups of
five bulls were killed at 34 wk of age, puberty (first ejaculate containing 50 x 106 sperm;
10% progressively motile) and 10, 20, or 30 wk after puberty. Three adult bulls also
were studied. Droplets of neutral lipid were present throughout the epithelium in all
bulls, but they were most numerous in the mid and distal regions of the caput epididymidis.
The epithelia in these regions were devoid of CPL. In the proximal caput epididymidis,
however, the intensity of staining for CPL increased from negative or weak in 34 wk
and puberty bulls to moderate or intense in older bulls. In the epithelium of the proximal
and distal corpus epididymidis, staining for CPL generally was moderate for all bulls.
Staining for CPL in the proximal cauda epididymidis declined from moderate or intense
in 34 wk and puberty bulls to virtual absence of staining in the oldest bulls. The changes
in staining intensity and distribution of CPL probably reflect modifications in the secretory
and absorptive functions of the epididymal epithelium during the first 30 wk after puberty.
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