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Biology of Reproduction, Vol 11, 198-204, Copyright © 1974 by Society for the Study of Reproduction

Histochemical Localization of Neutral Lipid and Choline-containing Phospholipid in the Epididymides of Neopuberal Holstein Bulls

G. J. KILLIAN 1, and R. P. AMANN 1

1 Dairy Breeding Research Center, The Pennsylvania State University, University Park, Pennsylvania 16802


Postpuberal changes in the distribution of neutral lipid and choline-containing phospholipid (CPL) in the epididymal epithelium were evaluated histochemically. Groups of five bulls were killed at 34 wk of age, puberty (first ejaculate containing 50 x 106 sperm; ge10% progressively motile) and 10, 20, or 30 wk after puberty. Three adult bulls also were studied. Droplets of neutral lipid were present throughout the epithelium in all bulls, but they were most numerous in the mid and distal regions of the caput epididymidis. The epithelia in these regions were devoid of CPL. In the proximal caput epididymidis, however, the intensity of staining for CPL increased from negative or weak in 34 wk and puberty bulls to moderate or intense in older bulls. In the epithelium of the proximal and distal corpus epididymidis, staining for CPL generally was moderate for all bulls. Staining for CPL in the proximal cauda epididymidis declined from moderate or intense in 34 wk and puberty bulls to virtual absence of staining in the oldest bulls. The changes in staining intensity and distribution of CPL probably reflect modifications in the secretory and absorptive functions of the epididymal epithelium during the first 30 wk after puberty.

Accepted on March 5, 1974




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R.L. Gerena, D. Irikura, N. Eguchi, Y. Urade, and G.J. Killian
Immunocytochemical Localization of Lipocalin-Type Prostaglandin D Synthase in the Bull Testis and Epididymis and on Ejaculated Sperm
Biol Reprod, March 1, 2000; 62(3): 547 - 556.
[Abstract] [Full Text]




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Copyright © 1974 by the Society for the Study of Reproduction.