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Biology of Reproduction, Vol 11, 504-508, Copyright © 1974 by Society for the Study of Reproduction
-Galactosidase in Rat Ovarian Bursa Fluid at Ovulation
1 Laboratories of Human Reproduction and Reproductive Biology and The Dept. of
Anatomy, Harvard Medical School, 45 Shattuck Street, Boston, Massachusetts 02115
-Galactosidase activity was assayed fluorometrically in the fluid which accumulates
within the ovarian bursa of the rat at the time of ovulation. Its concentration there was
found to be 7.5-fold greater than its concentration in plasma. The enzyme is a typical
acid hydrolase, presumed to occur in lysosomes in cells of the ovarian follicle wall. Its
accumulation in bursa fluid suggests that lysosomal enzymes may be released from follicle
walls during ovulation and washed into the bursa by the exudation of fluid through the
surface of follicles.
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