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Biology of Reproduction, Vol 11, 553-557, Copyright © 1974 by Society for the Study of Reproduction

The Effect of Low Concentrations of [3H]Thymidine on Pre- and Postimplantation Mouse Embryos

DON HORNER 1, and ANNE McLAREN 1

1 Dept. of Genetics, University of Edinburgh, Edinburgh, Scotland EH9 3JN


The effect of low concentrations of [3H]thymidine was studied on mouse blastocysts cultured in vitro. Experimental concentrations of 1 nCi/ml and 5 nCi/ml [3H] thymidine were shown to have no effect on the number of blastomeres or the rate of development up to the blastocyst stage. In blastocysts cultured in the higher [3H]thymidine concentration, mitotic rate appeared to be lower in the trophoblast than in the inner cell mass.

Blastocysts cultured under the experimental conditions were transferred to the uterine horns of pseudopregnant mice of the same strain. Histologic examination of the postimplantation embryos showed normal development of embryos cultured in 1 nCi/ml [3H]thymidine, and under these conditions fertile adults were produced. Embryos cultured in 5 nCi/ml [3H]thymidine sometimes induced a decidual reaction but never underwent normal implantation and development.

 Accepted on July 23, 1974






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Copyright © 1974 by the Society for the Study of Reproduction.