Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 11, 566-577, Copyright © 1974 by Society for the Study of Reproduction

Estrogen Cytosol Binding Proteins in Bovine Endometrium and Corpus Luteum

FRANCES A. KIMBALL 1, and WILLIAM HANSEL 1

1 Division of Biological Sciences and the Department of Animal Science, Cornell University, Ithaca, New York 14850


Estrogen binding by endometrial and luteal cytosol proteins was determined by a dextran—charcoal adsorption method and correlated with plasma hormone levels in the same animals. Cytosol preparations from both tissues demonstrated saturable high affinity (10-9M) binding of estradiol. Inhibition of endometrial protein binding of [3H]estradiol-17beta was obtained with estradiol-17beta> estrone> estradiol-17agr> diethylstilbestrol at 0°C. Binding by luteal protein could be inhibited with estradiol-17beta> estrone. Estradiol-17beta binding could not be inhibited with progesterone or cortisol. The concentration (pmole/mg protein) of estrogen-binding protein increased (p < .025) in the endometrium between the fifteenth and twenty-first days of the estrous cycle when compared to the second, fifth, and tenth days. The concentration of binding protein (on Day 15) was highly correlated with the estradiol-17beta and estrone plasma levels found at that time (p < 0.01). Luteal estrogen-binding protein concentration increased from Day 5 to Day 10 (p < 0.01) and remained at the same level through Day 15. There was a significant decline in binding protein concentration at Days 17-18, at the initiation of luteal regression, followed by an increase in concentration of luteal-binding protein at Day 21 to a level higher than at any other time during the cycle. Plasma progesterone and estrone levels declined at Days 17-18, while plasma estradiol levels increased. Ovariectomy resulted in an increase in the concentration of endometrial estrogen binding protein over that in intact heifers. Estrogen treatment of ovariectomized heifers caused a decrease in the concentration and content of binding protein 3 h after treatment. Eighteen hours after treatment the concentration was depressed but the total uterine content was increased.

Accepted on July 23, 1974




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Copyright © 1974 by the Society for the Study of Reproduction.