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Biology of Reproduction, Vol 12, 284-288, Copyright © 1975 by Society for the Study of Reproduction

Ovulation and Luteal Function in the Rabbit in Response to Injection or Infusion of Synthetic Gonadotropin-Releasing Hormone (Gn-RH)

GEORGE R. FOXCROFT 1, JOHN W. HAMILTON 1, , and ANDREW V. NALBANDOV 1

1 Animal Genetics-Animal Science, University of Illinois, Urbana, Illinois 61801


The potential of different treatments of synthetic gonadtropin-releasing hormone (Gn-RH) to stimulate ovulation in the adult female rabbit was investigated. Doses of 1.0-20.0 µg Gn-RH were administered either as a single iv injection or 60-min infusion (0.9% NaCl being used in controls) and all animals were subsequently inseminated. Both the number of females that ovulated and the mean number of ovulations were related to the dose of Gn-RH administered. This dose relationship was more predictable following injection than following infusion of releasing hormone. There were no significant treatment effects on the viability of ova 48 h after Gn-RH treatment. Injection of 50.0 or 100.0 µg Gn-RH, even following natural mating, did not result in superovulation. Measurement of CL weights and ovarian vein progesterone (P4) outflow 9 days after Gn-RH-induced ovulation revealed a significant positive relationship between CL weight and dose of Gn-RH injected or infused. No comparable effect on luteal P4 output was established.

Accepted on September 24, 1974






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Copyright © 1975 by the Society for the Study of Reproduction.