Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 12, 325-328, Copyright © 1975 by Society for the Study of Reproduction

Respiratory and Phosphorylative Activity of Amphibian Spermatozoa

ALBERTO G. DEL RIO 1, ANGEL M. ORCE REMIS 1, OSCAR A. ROVERI 2, , and RUBEN H. VALLEJOS 2

1 Instituto de Biologia, Universidad Nacional de Tucumán, Argentina
2 Departamento de Bioquimica. Facultad de Ciencias Bioquimicas, Universidad Nacional de Rosario. Rosario, Argentina


Bufo arenarum or Leptodactylus chaquensis spermatozoa have an endogenous respiration (Z002 = 1.4 -4.2) that was not significantly enhanced by glucose or fructose. Succinate was oxidized by these spermatozoa at higher rates (Z002 = 5-14) while endogenous respiration was only slightly stimulated by glutamate or citrate.

Both the endogenous and exogenous respiration were sensitive to respiratory chain inhibitors (rotenone, antimycin or cyanide).

The respiration of frog and toad sperm was coupled to the synthesis of ATP. P/O ratios of 0.54 were determined in the presence of potassium fluoride and exogenous hexokinase. Oligomycin inhibited phosphorylation completely and respiration partially. The latter inhibition was released by uncouplers like dinitrophenol.

It is concluded that frog and toad sperm have an active oxidative metabolism coupled to the synthesis of ATP.

 Accepted on October 1, 1974






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Copyright © 1975 by the Society for the Study of Reproduction.