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Biology of Reproduction, Vol 12, 393-395, Copyright © 1975 by Society for the Study of Reproduction
1 National Dairy Research Institute, Karnal, India Phospholipids of goat spermatozoa and seminal plasma were separated and quantified by two
dimensional thin layer chromatography. The spermatozoal phospholipids were comprised of 25.0
percent phosphatidyl choline, 27.8 percent phosphatidal choline (choline plasmalogen). 5.0 percent
phosphatidyl ethanolamine, 0.9 phosphatidal ethanolamine (ethanolamine phasmalogen), 11.8 percent sphingomyelin, 2.8 percent phosphatidyl serine, 1.8 percent phosphatidyl inositol, 4.4 percent
lysophosphatidyl choline. 4.3 percent lysophosphatidyl ethanolamine, 8.0 percent diphosphatidyl
glycerol (cardiolipin) and 0.5 percent phosphatidic acid. The corresponding values for the seminal
plasma phospholipids were 18.3, 19.1, 9.1, 3.0, 15.0, 4.1, 3.5, 5.5, 9.6, 2.0 and 0.7 percent, respectively.
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