Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 12, 415-422, Copyright © 1975 by Society for the Study of Reproduction

Purification of Turkey Gonadotropins

SUSAN WALKER FARMER 1, HAROLD PAPKOFF 1, , and PAUL LICHT 1

1 Hormone Research Laboratory, University of California, San Francisco, California 94143 and Department Zoology, University of California, Berkeley, california 94720


Turkey pituitaries, like mammalian pituitaries, were found to contain two gonadotropic factors. Highly purified LH was prepared and partially characterized. A potent turkey FSH was also prepared. Both gonadotropins were found to be similar to mammalian, turtle, and frog gonadotropins in terms of chromatographic behavior and the presence of carbohydrate. The turkey LH was found to be similar to ovine LH in amino acid composition but to contain significantly less carbohydrate than ovine LH. The sedimentation behavior of the turkey LH in neutral and acidic solutions suggested that this material is composed of subunits. Turkey gonadotropins appear to be more potent when assayed in non-mamalian systems than when tested in standard mammalian bioassays for LH and FSH.

Accepted on November 12, 1974






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Copyright © 1975 by the Society for the Study of Reproduction.