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Biology of Reproduction, Vol 12, 641-646, Copyright © 1975 by Society for the Study of Reproduction
1 Departments of Medicine and Biochemistry, Indiana University School of Medicine,
Indianapolis, Indiana 46202 The glutathione and thiol contents of spermatozoa and seminal plasma of several animal species have
been examined. Washed, ejaculated or epididymal spermatozoa from man, dog, ram and goat were
found to contain about 5 (1-13) nmoles of glutathione per 10## cells. Little or none was present in rabbit
and boar spermatozoa. In contrast, the total thiol content of the sperm samples was 350 (95-700)
nmoles/10## cells, but almost all of it was protein-bound. Dog and human seminal plasma similarly
contained thiol groups which were almost entirely protein-bound. The concentration of glutathione in
seminal plasma was less than 2 µM. The spermatozoa which contained glutathione also exhibited
substantial glutathione reductase and glutathione peroxidase activities. These findings suggest the
possibility that these enzymes and glutathione may be functional in the spermatozoa of some animals as
a protective mechanism against oxidative damage by H2O2, as has been demonstrated in other cells.
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