Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 12, 657-663, Copyright © 1975 by Society for the Study of Reproduction

Esterification of Cholesterol by Rabbit Testicular Mitochondria

J. P. RENSTON 1, T. J. IHRIG 1, R. H. RENSTON 1, B. GONDOS 1, , and R. J. MORIN 1

1 Department of Pathology and Reproductive Endocrinology Center, University of California School of Medicine, San Francisco, California 94143 and Department of Pathology, Harbor General Hospital, UCLA School of Medicine, Torrance, California 90509


The characteristics of a cholesterol esterifying enzyme in rabbit testicular mitochondria have been investigated. Cholesterol esterification with (1-14C) palmitic acid occurred in vitro via a probable sterol esterase reaction. Two peak rates of enzyme activity were observed, one in the region of pH 3.8 and another, much smaller peak rate, in the region of pH 5.4. Esterification was linearly related to time up to 4-5 h of incubation and increased linearly with increasing mitochondrial protein concentration up to 50 µg per incubation. Esterifying activity was stimulated with increasing incubation temperature between 29°C and 41°C. Sterol esterase activity in the rabbit testis may function to accumulate a precursor pool of cholesterol to be subsequently utilized in steroidogenesis.

Accepted on February 19, 1975






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Copyright © 1975 by the Society for the Study of Reproduction.