Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by RUSSO, J.
Right arrow Articles by DUNBAR, B. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by RUSSO, J.
Right arrow Articles by DUNBAR, B. S.
Agricola
Right arrow Articles by RUSSO, J.
Right arrow Articles by DUNBAR, B. S.

Biology of Reproduction, Vol 13, 136-141, Copyright © 1975 by Society for the Study of Reproduction

Membrane Damage to Immunologically Immobilized Rabbit Spermatozoa Visualized by Immunofluorescence and Scanning Electron Microscopy

JOSE RUSSO 1, CHARLES B. METZ 1, , and BONNIE S. DUNBAR 1

1 Institute for Molecular and Cellular Evolution, University of Miami, Coral Gables, Florida 33134


Rabbit sperm fluoresced relatively uniformly following treatment with goat or rabbit antirabbit semen globulin alone or in combination with heated complement. However, following an immobilizing treatment, namely, fluorescein conjugated goat or rabbit antirabbit semen globulin plus active C', the sperm fluoresced minimally in the acrosomal region but brightly in the post acrosomal area. This appearance corresponds to the position of the cell membrane of the sperm head following the rolling back of the cell membrane seen by transmission electron microscopy (Russo and Metz, 1974). Comparable posterior fluorescence was observed by indirect immunofluorescence. This posterior fluorescent pattern may be diagnostic for immunologically immobilized rabbit sperm.

Membrane damage associated with immunological immobilization was also seen with SEM. This appeared as an accumulation of material interpretable as a "rolling back" of the cell membrane from the acrosomal apex.

 Submitted on November 25, 1974
Accepted on April 17, 1975






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the Society for the Study of Reproduction.