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Biology of Reproduction, Vol 13, 274-281, Copyright © 1975 by Society for the Study of Reproduction

Studies on the Nature of Extracellular Components of Rat Seminiferous Tubular Wall. I. Isolation and Chemical Characterization of Basement Membrane

BERTA DENDUCHIS 1, LIVIA LUSTIG 1, NELIDA N. GONZALEZ 1, , and ROBERTO E. MANCINI 1

1 IIa Catedra de Histologia—Centro de Investigaciones en Reproduccion Paraguay 2l55-10 Piso, Facultad de Medicina, Buenos Aires, Argentina


In an attempt to isolate the seminiferous tubule basement membrane from testes of normal rats, a preparation (STBM) was obtained by a method involving the use of sieves, sonication and differential centrifugation. Electron microscopic studies show that this material is mainly composed of basement membranes. A low proportion of collagen fibrils and some cellular debris are also present.

The nitrogen content, the presence of sugars and the very low amount of lipids suggest that the preparation is mostly glycoprotein. Moreover, STBM is characterized by large amounts of glycine, hydroxyproline and hydroxylysine (199, 66 and 16 residues per 1000 residues of total amino acids, respectively) and the presence of 5.8 percent of carbohydrate composed of glucose, galactose, mannose, fucose, hexosamines and sialic acid. The amino acid and carbohydrate composition resemble that of other isolated basement membranes.

 Submitted on July 1, 1974
Accepted on May 9, 1975






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Copyright © 1975 by the Society for the Study of Reproduction.