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Biology of Reproduction, Vol 13, 322-328, Copyright © 1975 by Society for the Study of Reproduction
1 Reproduction Laboratory
Animal Physiology and Genetics Institute, USDA
Agricultural Research Center
Beltsville, Maryland 20705 One hundred parous ewes were assigned to the following five groups, and each ewe was mated
naturally during estrus: 1) untreated controls; 2) treated by subcutaneous injection with 20 µg of
estradiol-17 Ten ewes from each group were killed at 2 h and ten at 22 h after mating. The cervix (divided
into three segments of equal length), uterus and oviducts were flushed and sperm cells counted. At
2 h after mating, the numbers of sperm cells recovered from the anterior cervix (geometric means
in millions for Groups 1 through 5, respectively) were 2.3, 2.5, 0.3, 0.3 and 0.2 (P<0.01). At 22 h,
the numbers (geometric means in thousands) recovered from the oviducts were 16.5, 6.7, 0.5, 0.3
and 0.1 (P<0.01). In three groups of ewes, partial inhibition of sperm transport into the anterior
cervix was followed by partial failure of sperm transport into the oviducts.
at the time of mating; 3) treated on Day 10 of an estrous cycle with 10 mg of
prostaglandin F2
(PG) to cause premature regression of corpora (ewes were in estrus and were
mated on Day 12); 4) treated during an estrous cycle with 60 mg of medroxyprogesterone acetate
(MAP) in an intravaginal sponge inserted at Day 8 and removed at Day 25 (ewes were in estrus and
were mated on Day 27 or 28); 5) treated with a plastic spiral intrauterine device (IUD) inserted by
surgery into one uterine horn (ewes were mated at an estrus occurring at least 2 wk after surgery).
Accepted on June 16, 1975
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