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Biology of Reproduction, Vol 14, 157-162, Copyright © 1976 by Society for the Study of Reproduction

Successful Culture in vitro of Bovine Embryos to the Blastocyst Stage

RAYMOND W. WRIGHT JR. 1, GARY B. ANDERSON 1, PERRY T. CUPPS 1, , and MAARTEN DROST 1

1 Department of Animal Science, University of California, Davis, California 95616


Two hundred and sixty bovine embryos between the two- and eight-cell stage were collected from synchronized, PMSG-treated cows. Embryos were placed randomly in culture among 11 media and 2 gas atmospheres and development was recorded at 24 h intervals for the duration of culture. The number of cells present at the cessation of culture was determined following aceto-orcein staining.

Ham's F-1O medium supplemented with heat treated fetal calf serum (HTFC), supported the development of 9 of 43 (21 percent) eight-cell embryos into expanded blastocysts after 134 to 150 h in culture and 1 of the 9 expanded blastocysts appeared to undergo an in vitro hatching process after 160 h in culture. No other media tested supported development of bovine embryos beyond the early blastocyst stage (32 to 64 cells). The reduced oxygen atmosphere of 90 percent N2, 5 percent CO2, 5 percent O2 was superior (P<0.01) to 5 percent CO2 in air in both the number of embryos undergoing division and in degree of development.

Ham's F-10 medium supplemented with HTFC serum under a 90 percent N2, 5 percent CO2, 5 percent O2 gas atmosphere successfully supported the development of four-, six- and eight-cell bovine embryos cultured in vitro.

 Submitted on July 8, 1975
Accepted on October 2, 1975




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Copyright © 1976 by the Society for the Study of Reproduction.