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Biology of Reproduction, Vol 14, 194-201, Copyright © 1976 by Society for the Study of Reproduction

Effects of Mammalian and Nonmammalian Gonadotropins on Androgen Production by Minced Rabbit Testis

PAUL LICHT 1, CHARLES H. MULLER 1, , and HING WO TSUI 1

1 Department of Zoology, University of California, Berkeley, California 94720


Androgen production by minced testis from adult rabbits was measured in response to a variety of FSHs and LHs from eutherian (sheep, rat, rabbit) and metatherian (kangaroo) mammals, a bird (turkey), reptiles (turtle, alligator) and an amphibian (frog). LHs from all three eutherian species were highly potent and produced clear stimulation at doses below 50 ng/ml; relative potencies in the rabbit were consistent with estimates from OAAD assay, but an anuran ovulation assay indicated considerable differences in potency among these three preparations. LH from the kangaroo had only about 4 percent the activity of the eutherian LHs in the rabbit testis. Potencies of mammalian (rat and ovine) FSHs were only a small fraction of the LHs (<1 percent); inactivation of ovine FSH by an LH-antiserum indicated that this low level of activity was due to residual LH contamination.

Amphibian, reptilian and avian hormones were active in the rabbit testis, but a sharp dichotomy in potency was evident between these and all mammalian hormones. Nonmammalian hormones stimulated androgen production (with comparable ratios of testosterone and dihydrotestosterone), and maximal levels of androgen produced were the same or greater than obtained with mammalian preparations. However, nonmammalian hormones were consistently three to four orders of magnitude less potent than the eutherian LHs. Furthermore, in several tests, nonmammalian FSHs were also active in the minced rabbit testis at levels (20-110 percent of homologous LH) that could not be readily attributed to LH contamination. Comparisons with relative potencies for the same preparations in an anuran ovulation assay confirm that pronounced phylogenetic specificity underlies the differences in potencies between mammalian and nonmammalian gonadotropins.

Note:
ACKNOWLEDGMENTS We are indebted to Drs. Harold Papkoff and Susan Farmer for help in preparing and assaying various mammalian and nonmammalian hormones and to the NIAMD and its rat hormone distribution program for the supply and bioassay data on various mammalian hormones. Dr. Bruce Goldman provided antiserum against mammalian LH. Dr. Gerald Connell offered invaluable advice and assistance in helping us establish the rabbit testis system. Mr. Hugh Meakin provided technical assistance. This work was supported in part by NSF grant GB 3521X. CUM was supported by an NIH training grant, Ca 05045.

Submitted on May 28, 1975
Accepted on October 9, 1975




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Copyright © 1976 by the Society for the Study of Reproduction.