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Biology of Reproduction, Vol 14, 398-400, Copyright © 1976 by Society for the Study of Reproduction

Tepa—A Marker for use in Evaluating Fertilizing Ability of Rabbit Spermatozoa in Competitive Fertilization

RICHARD E. BEIL 1, DOREEN A. BAUMAN 1, , and CHARLES N. GRAVES 1

1 Department of Dairy Science, University of Illinois, Urbana, Illinois 61801


Rabbit spermatozoa were exposed to the alkylating agent tris-(1-aziridinyl)-phosphine oxide (TEPA) prior to insemination into females. Eggs fertilized with TEPA treated spermatozoa did not develop beyond the 2-3-cell stage whereas eggs fertilized with control spermatozoa developed normally. TEPA treated spermatozoa successfully competed for fertilization of ova with control spermatozoa. Thus rabbit spermatozoa can be marked with TEPA for studies involving fertility comparisons of populations of spermatozoa.

Note:
ACKNOWLEDGMENT This research was supported by funds from the Agricultural Experiment Station at the University of Illinois.

Submitted on October 22, 1975
Accepted on December 4, 1975






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Copyright © 1976 by the Society for the Study of Reproduction.