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Biology of Reproduction, Vol 14, 416-420, Copyright © 1976 by Society for the Study of Reproduction

The Plasma Level of 5agr-DHP After Intramuscular Injection of 5agr-DHP and the Induction of Uteroglobin in Ovariectomized Rabbits

SHEIKH SAIF UR RAHMAN 1, R. B. BILLIAR 1, , and B. LITTLE 1

1 The Department of Reproductive Biology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106


A competitive protein-binding assay for the determination of 5agr-pregnan-3, 20-dione (5agr-DHP) in rabbit plasma is described. It is based on the specificity of the progesterone binding plasma protein (PBP) of the pregnant guinea pig. This method was applied to plasma samples obtained from ovariectomized rabbits which had received intramuscular injections of oil vehicle or 2 mg/kg bodyweight of 5agr-DHP. The intramuscular injection of 5agr-DHP resulted in plasma levels of 5agr-DHP about one-tenth those of progesterone after the I.M. injection of progesterone. Two mg/kg bodyweight of 5agr-DHP and 0.2 mg/kg bodyweight of progesterone resulted in similar blood concentration of each steroid (3-10 ng/ml). This concentration of progesterone, but not 5agr-DHP, induced uteroglobin in the uterus. Therefore, it is concluded that 5agr-DHP does not mediate progesterone induction of uteroglobin in the rabbit uterus.

Note:
ACKNOWLEDGMENTS The authors wish to acknowledge the technical assistance of Mr. Augusts Heinsons and Mrs. Anna Juang during the course of this work.

Submitted on September 4, 1975
Accepted on December 16, 1975






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Copyright © 1976 by the Society for the Study of Reproduction.