Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MERCADO, E.
Right arrow Articles by ROSADO, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MERCADO, E.
Right arrow Articles by ROSADO, A.
Agricola
Right arrow Articles by MERCADO, E.
Right arrow Articles by ROSADO, A.

Biology of Reproduction, Vol 14, 632-640, Copyright © 1976 by Society for the Study of Reproduction

Sulfhydryl Groups on the Spermatozoa Membrane. A Study with a New Fluorescent Probe for SH Group

EFRAIN MERCADO 1, GUILLERMO CARVAJAL 1, ALEJANDRO REYES 1, , and ADOLFO ROSADO 1

1 Sección de Bioquimica de la Reproduccioón, Departamento de Investigacioón en Medicina, Experimental, Instituto Mexicano del Seguro, Social, Apdo. Postal 73-032, México 73, D. F.


We report the properties of a new sulthydryl reagent, N[unknown] (4-carboxy-3-hydroxy-phenyl) maleimide (NCPM), which forms photostable, covalent fluorescent adducts with organic compounds, proteins and biological membranes containing sulfhydryl groups. NCPM appears not to permeate cell membranes and, since its reaction with SH compounds is stoichiometric, permits the quantitative assessment of the exposed functional groups on the surface of cells. Binding of NCPM to washed spermatozoa resulted in a considerable increase in fluorescence that was linearly related to the number of cells. Under similar experimental conditions rabbit sperm cells showed a significantly smaller binding of NCPM than human sperm cells (35 ± 5.2 against 55 ± 10 nmoles of SH groups/108 cells). This difference was enhanced in the presence of EDTA, which stimulated binding. Five times as high a concentration of SH groups was detected on the membrane of the sperm cells as on erythrocyte ghosts. In addition, while on the latter, most of the SH groups (85 percent) not readily reactive with NCPM, almost all of those present on the sperm cells are readily available.

Submitted on August 26, 1976
Accepted on February 18, 1976




This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
J. Zhang, J. Wu, R. Huo, Y. Mao, Y. Lu, X. Guo, J. Liu, Z. Zhou, X. Huang, and J. Sha
ERp57 is a potential biomarker for human fertilization capability
Mol. Hum. Reprod., September 1, 2007; 13(9): 633 - 639.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the Society for the Study of Reproduction.