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Biology of Reproduction, Vol 15, 66-78, Copyright © 1976 by Society for the Study of Reproduction
1 Department of Physiology and Biophysics,
Colorado State University,
Fort Collins, Colorado 80523 Intra-uterine administration of prostaglandin F2
-tham salt (PGF2) to ewes on Days 9 or 10
of the estrous cycle was followed by reduced flow of blood to the ovary containing the corpus
luteum and reduced levels of progesterone in the systemic circulation. These parameters were
highly correlated (P<0.01). In an attempt to separate a direct effect of PG on the luteal cell from
the effects on ovarian blood flow, analogs of PGF2, ICI-79939 and ICI-81008, having 10 times
more and 50 times less smooth muscle stimulating activity than PGF2, respectively, were
administered intramuscularly to ewes on Day 10 of the estrous cycle. Levels of progesterone in the
systemic circulation and blood flow to the ovary with a corpus luteum decreased to basal levels
within 12 h after treatment in both groups of ewes. Ovarian blood flow and systemic progesterone
levels were also correlated in ewes receiving ICI-79939 (r = 0.87, P<0.01) and ICI-81008 (r = 0.50,
P<0.05) In corpora lutea removed from ewes 0, 6, 12, 24 and 48 h after administration of PGF2
there was a decrease in percentage of the total volume of the corpus luteum occupied by
endothelial cells (P<0.001), red blood cells (P<0.01) and lumens of capillaries (P<0.05) with time.
There was an increase in cellular debris in the extra-cellular space (P<0.01) and within capillaries
(P<0.05) after injection of PGF2. Percent volume of the corpus luteum occupied by theca-lutein
cells, fibroblasts, eosinophils, connective tissue and white blood cells was not affected by this
treatment. Ultrastructurally, corpora lutea removed from ewes 6 h after injection of PGF2 were
similar to corpora lutea removed from control ewes. By 12 h, a proliferation of coated vesicles,
dense bodies and lipid droplets was observed in granulosa-lutein cells and local areas of cytoplasmic
degeneration were visible. By 24 h after treatment with PGF2 many of the capillaries which were
present contained cellular debris, nucleoli in granulosa-lutein cells were rare and granules within the
matrix of the mitochondria were numerous. By 48 h, granulosa-lutein cells were extremely
shrunken, contained irregular nuclei with condensed chromatin, abundant lipid droplets and
swollen mitochondria. Morphological changes occurring in the theca-lutein cells after administration of PGF2 to ewes were similar to the changes described for granulosa-lutein cells. The data
obtained from these experiments indicate that administration of PGF2 to ewes reduced blood
flow to the ovary with a corpus luteum and reduced secretion of progesterone. Morphological
studies suggested that PGF2 affected the vascular component of the corpus luteum.
Accepted on March 13, 1976
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