Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 15, 187-194, Copyright © 1976 by Society for the Study of Reproduction

The Extraction of Acrosin from Human Spermatozoa

PETER S. TOBIAS 1, and GEBHARD F. B. SCHUMACHER 1

1 Laboratory for Reproductive Biochemistry and Immunology, Department of Obstetrics and Gynecology, University of Chicago Pritzker School of Medicine, Chicago, Illinois 60637


Two methods for the extraction of acrosin from ejaculated human spermatozoa have been compared. Method 1 consists of the successive extraction of washed spermatozoa first with water, second with a mixture of Hyamine and Triton X-100, and third with dilute acetic acid. Method 2 consists of the extraction of saline washed spermatozoa with dilute acetic acid. Method 1 yields nearly twice as much acrosin of a higher specific activity than Method 2. Method 2 yields a single band of amidase activity upon polyacrylamide electrophoresis. Method 1 also yields two additional bands of amidase activity.

Note:
ACKNOWLEDGMENT We are grateful for the excellent technical assistance of Mr. M. B. Dragoje. This work was supported in part by the World Health Organization Project #73008, NIH Grant #HD-06315, Ford Foundation Grant #690-0108A and the Biomedical Center for Population Research Grant #NIH-HD-07110.

Submitted on February 4, 1976
Accepted on April 20, 1976






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Copyright © 1976 by the Society for the Study of Reproduction.