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Biology of Reproduction, Vol 15, 260-265, Copyright © 1976 by Society for the Study of Reproduction

Chromatographic Separation of Puberty Accelerating Pheromone from Male Mouse Urine

JOHN G. VANDENBERGH 1, J. S. FINLAYSON 2, WALTER J. DOBROGOSZ 3, STEVEN S. DILLS 3, , and THOMAS A. KOST 3

1 N. C. Department of Mental Health, Research Division, Raleigh, North Carolina
2 Bureau of Biologics, Food and Drug Administration, Bethesda, Maryland
3 Department of Microbiology, N. C. State University, Raleigh, North Carolina


Dialysis and sequential ultrafiltration were used to separate urine from adult male mice into a number of fractions, all of which proved to be capable of accelerating the sexual maturation of female mice. Chromatography of the lowest molecular weight fraction on Sephadex G-15 yielded several fractions, only one of which was active. The active fraction was eluted at a position corresponding to a molecular weight of 860. It contained no detectable steroids but gave a strong positive test for peptides.

Submitted on March 16, 1976
Accepted on May 11, 1976




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