Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WU, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WU, J. T.
Agricola
Right arrow Articles by WU, J. T.

Biology of Reproduction, Vol 15, 350-355, Copyright © 1976 by Society for the Study of Reproduction

Cell Loss of the Aged Rat Blastocysts in Relation to Their Viability

J. T. WU 1

1 Worcester Foundation for Experimental Biology Shrewsbury, Massachusetts 01545


The number of cells in the trophoblast and inner cell mass (ICM) were counted in delayed blastocysts recovered from hypophysectomized or ovariectomized rats treated with progesterone.

In the trophoblast the number of cells declined steadily from the 90s on Day 9 to the low 70s on Day 52 (P<0.01) as did the whole blastocyst, from about 110 to 80 cells (P<0.01). In both cases no difference was observed between the blastocysts recovered from hypophysectomized and those from ovariectomized rats.

In the ICM the number of cells reduced abruptly (P<0.01) between Day 9 (17.6 cells) and Day 20 (9.3 cells) in hypophysectomized rats, but not until Day 20 (14.9 cells) and Day 32 (8.6 cells) in ovariectomized rats. In each case no further decrease was noted.

Thus, the reduction of cell numbers in the ICM appears to be proportionally more drastic than in the trophoblast. The difference in the number of ICM cells of the blastocyst on Day 20 between the hypophysectomized and the ovariectomized rats appears to be parallel with their viability (23 and 52 percent, respectively). However, the further decrease in blastocyst viability on Day 32 and beyond may be related to the dispersion, rather than the number, of ICM cells.

Note:
ACKNOWLEDGMENTS This study was supported by grants from NIH (HD 03003) and the Ford Foundation, both awarded to M. C. Chang whose interest and guidance in this study are greatly appreciated.

Submitted on March 5, 1976
Accepted on May 18, 1976






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1976 by the Society for the Study of Reproduction.