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Biology of Reproduction, Vol 15, 467-470, Copyright © 1976 by Society for the Study of Reproduction
1 Department of Anatomy and Reproductive Biology,
University of Hawaii School of Medicine,
Honolulu, Hawaii 96822 Isolated nuclei of hamster spermatozoa develop into male pronuclei when injected into hamster
eggs. The nuclei of fresh, frozen-thawed and freeze-dried human spermatozoa are equally capable
of developing into male pronuclei when injected into hamster eggs. These results indicate that
sperm nuclei are stable organelles and the egg cytoplasmic factors controlling the transformation of
sperm nuclei into male pronuclei are not strictly species-specific.
Note:
ACKNOWLEDGMENTS
This work was supported by grants from the
Population Council and the Ford Foundation and
a University of Hawaii Intramural Research grant.
We thank Ms. C. A. Mahi and Dr. B. J. Rogers for
their assistance in the preparation of the manuscript.
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