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Biology of Reproduction, Vol 15, 467-470, Copyright © 1976 by Society for the Study of Reproduction

Microsurgical Injection of Spermatozoa into Hamster Eggs with Subsequent Transformation of Sperm Nuclei into Male Pronuclei

T. UEHARA 1, and R. YANAGIMACHI 1

1 Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822


Isolated nuclei of hamster spermatozoa develop into male pronuclei when injected into hamster eggs. The nuclei of fresh, frozen-thawed and freeze-dried human spermatozoa are equally capable of developing into male pronuclei when injected into hamster eggs. These results indicate that sperm nuclei are stable organelles and the egg cytoplasmic factors controlling the transformation of sperm nuclei into male pronuclei are not strictly species-specific.

Note:
ACKNOWLEDGMENTS This work was supported by grants from the Population Council and the Ford Foundation and a University of Hawaii Intramural Research grant. We thank Ms. C. A. Mahi and Dr. B. J. Rogers for their assistance in the preparation of the manuscript.

Submitted on May 14, 1976
Accepted on June 29, 1976




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Copyright © 1976 by the Society for the Study of Reproduction.