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Biology of Reproduction, Vol 15, 579-585, Copyright © 1976 by Society for the Study of Reproduction

Comparative Study of Cholinesterases Distribution in the Spermatozoa of Some Mammalian Species

JYOTSNA CHAKRABORTY 1, and LEONARD NELSON 1

1 Department of Physiology, Medical College of Ohio, Toledo, Ohio 43614


The localization of both acetyl- and nonspecific cholinesterases has been demonstrated in the spermatozoa of six different mammalian species: human, chimpanzee, bull, rat, rabbit and mouse. The reaction sites were essentially the same in the spermatozoa of all six species. The main site of reaction was found to be the fibrillar component of the sperm tail. Some reactivity was also noticed in the mitochondrial membrane. Cytoplasmic droplets and some particulate matter in the seminal plasma were always reactive. A variety of substrates and inhibitors were used to distinguish between the acetyl- and nonspecific cholinesterases. It was found that the spermatozoa exhibit positive cytochemical reactions for both enzymes. Distinct variation in the amount of reaction product was observed in the spermatozoa of different species. Bull and rat spermatozoa exhibited maximum activity, rabbit and mouse spermatozoa were moderately reactive, while chimpanzee and human spermatozoa were least reactive.

Note:
ACKNOWLEDGMENTS The authors thank Miss Monica Young for her skillful technical assistance. Human semen was provided through the courtesy of Dr. J. Jhunjhunwala and Dr. K. Kropp, Urology Section of the Medical College of Ohio. Bull semen was generously supplied by Select Sires, Dr. H. Kelgrin and chimpanzee semen was obtained through the courtesy of Dr. C. Hardin, Toledo Zoological Park, Toledo, Ohio. This work was supported by USPHS Research Grant HD-03266 and GRS 94802.

Submitted on March 31, 1976
Accepted on July 27, 1976




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[Abstract] [Full Text] [PDF]


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Copyright © 1976 by the Society for the Study of Reproduction.