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Biology of Reproduction, Vol 15, 670-677, Copyright © 1976 by Society for the Study of Reproduction

Binding of Steroids by Intact Bovine Sperm

R. P. AMANN 1, and R. H. HAMMERSTEDT 1

1 Dairy Breeding Research Center and Department of Biochemistry-Biophysics, Pennsylvania State University, University Park, Pennsylvania 16802


A new filter assay technique was developed to evaluate spermatozoal binding at 5°C for steroid present at 0.1-5,000 nM. A charcoal-stripping technique was used to reduce the amount of endogenous steroid bound by sperm. Both stripped and non-stripped sperm bound steroids to essentially unsaturable sites. Progesterone was bound with greatest affinity followed by estradiol > dihydrotestosterone > testosterone > androsterone, androstenedione > androstanediol, etiocholanolone. We conclude that very few if any specific, saturable, high affinity steroid-binding sites exist on intact testicular or ejaculated sperm.

Note:
ACKNOWLEDGMENTS Drs. J. F. Kavanaugh and L. C. Griel, Jr. performed the surgery; Mrs. Sandra Hay and Mrs. Johanne Eastman provided skilled technical assistance.

Submitted on April 7, 1976
Accepted on August 18, 1976






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Copyright © 1976 by the Society for the Study of Reproduction.