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Biology of Reproduction, Vol 16, 564-570, Copyright © 1977 by Society for the Study of Reproduction

Mouse Blastocysts Pulse Labeled with 14C-Glucosamine: Incorporation and Ultrastructural Analysis

ROGER A. ACEY 1, LINDA D. HAZLETT 1, , and DANICA DABICH 1

1 Departments of Biochemistry and Anatomy, Wayne State University, School of Medicine, Detroit, Michigan 48201


In vitro pulse labeling of early mouse blastocysts (90 h p.c.) with 14C-glucosamine yielded linear time profiles for both total uptake of radioactivity and incorporation into acid precipitable material. Radioautographic fine structural analysis of pulse labeled embryos demonstrated distinct intra- and inter-cellular sites of labeling. Among these are precipitated fibrillar material in the blastocoel, trophoblast cell surfaces, lipid droplets, microtubules, Golgi complex, mitochondria, the nuclear envelope and nucleolar associated chromatin regions.

Note:
ACKNOWLEDGMENT These studies were supported by N.I.C.H.D. Grant number HD-06234 and N.I.H. Grant number RR-05384.

Submitted on November 22, 1976
Accepted on December 31, 1976




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Copyright © 1977 by the Society for the Study of Reproduction.