Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 17, 84-88, Copyright © 1977 by Society for the Study of Reproduction

Gamma-Glutamyl Transpeptidase Activity in the Developing Rat Testis. Enzyme Localization in Isolated Cell Types

CHERYLE LU 1, and ANNA STEINBERGER 1

1 Department of Reproductive Medicine and Biology, The University of Texas Medical School at Houston, Houston, Texas 77030


The purpose of this study was to correlate gamma glutamyl transpeptidase (ggr-GTP) activity with the morphological changes occurring in the rat testis during sexual maturation and to determine the cell type(s) responsible for the activity of this enzyme. The ggr-GTP activity was measured at different ages in whole testis homogenates, isolated specific cell types and cultured Sertoli cells. The specific activity of ggr-GTP, expressed as units per mg of protein (U/mg P), in whole testis homogenates increased with age of animals, reaching maximum value (0.60 ± 0.03 U/mg P) by the 20th day, thus, prior to the formation of spermatids, and then remained essentially unchanged until Day 100. When individual cell types were isolated from 30-day-old rat testis, the specific activities of ggr-GTP in the Sertoli cells and the germ cells respectively were 0.93 ± 0.07 U/mg P and 0.12 ± 0.05 U/mg P. The peritubular cells and interstitial cells contained non-detectable ggr-GTP activity. These results indicate that in 30-day-old rat testis the ggr-GTP activity is associated mainly with the Sertoli cells. The ggr-GTP activity of the isolated Sertoli cells was also investigated after various culture periods at 34°C. The specific activity of ggr-GTP in the cultured Sertoli cells increased with culture age, showing a 5-fold increase after three days of incubation and a 20-fold increase after seven days of culture.

The physiological significance of ggr-GTP activity in the Sertoli cells and its increase under culture conditions remain unclear, but the enzyme may play a role in the synthesis of specific proteins known to be secreted by the Sertoli cells.

Note:
ACKNOWLEDGMENT This work was supported in part by the National Institute of Health and Human Development Grant No. HD-08338.

Submitted on January 7, 1977
Accepted on March 1, 1977




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Copyright © 1977 by the Society for the Study of Reproduction.