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Biology of Reproduction, Vol 17, 269-288, Copyright © 1977 by Society for the Study of Reproduction
1 Sloan-Kettering Institute for Cancer Research,
425 East 68th Street,
New York, New York 10021 Spermiogenesis and the morphology of spermatozoa in sterile mice carrying 2 different lethal
t-haplotypes (T/t-locus) has been studied by transmission and scanning electron microscopy. In
mice heterozygous for the haplotype t0 and tw32 (genotype t0/tw32) nuclei of late spermatids fail
to elongate normally. Also, derrangements of the microtubules produce abnormal indentations of
the nucleus. Likewise acrosomes develop irregularities of shape which persist in spermatozoa.
Spermatozoa acquire additional abnormalities while they mature in the excurrent ducts.
Acrosomes frequently show extensive areas of separation from the nuclear membrane. In thin
sections, about 10 percent of spermatozoa from the vas deferens exhibit missing or misplaced
microtubules within the axoneme, defects that are absent in testicular and caput epididymal
spermatozoa. By SEM 60-75 percent of spermatozoa show abnormally short, angular or
amorphous heads. The irregularities of the shape of the acrosome can frequently be clearly viewed
as well as prominent bulges and ridges, probably reflecting areas of dehiscence of acrosome from
the nucleus. Defective chromosomal condensation may explain the abnormality of nuclear
elongation. Membrane defects may be responsible for the disorganized microtubules of the
manchette and for the failure of the acrosome to maintain a normal attachment to the nucleus.
Note:
ACKNOWLEDGMENTS
Work supported by NSF Grant BMS 16354 and
NIH Grants HD 10669-01 and CA 08748-12. The
authors thank Joyce Dieckmann and Stephen Dunn
for technical assistance.
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