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Biology of Reproduction, Vol 17, 350-354, Copyright © 1977 by Society for the Study of Reproduction

Induction of Zonal and Egg Plasma Membrane Blocks to Sperm Penetration in Mouse Eggs with Cortical Granule Exudate

DON P. WOLF 1, and MITSUMA HAMADA 1

1 Division of Reproductive Biology, Department of Obstetrics and Gynecology, and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19174


The contents of cortical granules (cortical granule exudate = CGE) were recovered from zona-free mouse eggs inseminated in vitro with capacitated epididymal spermatozoa. Preincubation of cumulus-free, zona-intact eggs in CGE led to reduced penetration levels upon insemination. This reduction was CGE concentration- and time-dependent and was susceptible to inhibition by soybean trypsin inhibitor and by p-aminobenzamidine. Control experiments eliminated active contributions from the sperm suspension used to elicit granule release. CGE was also active in reducing penetration of zona-free eggs. These results indicate that cortical granule contents are capable of modulating sperm penetration of mouse eggs at both the zona pellucida and at the egg plasma membrane.

Note:
ACKNOWLEDGMENTS The authors thank Ms. Pat Park for secretarial assistance and Dr. Edward Carroll, Jr. for a critical review of the manuscript. This work was supported by USPHS HD-07635 and Ford Foundation 65-58B grants.

Submitted on February 25, 1977
Accepted on April 27, 1977




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