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Biology of Reproduction, Vol 17, 432-442, Copyright © 1977 by Society for the Study of Reproduction

Composition and Synthesis of Gangliosides in Bovine Testis, Sperm and Seminal Plasma

ALFRED A. BUSHWAY 1, E. D. CLEGG 1, , and T. W. KEENAN 1

1 Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907


Gangliosides were identified as constituents of bovine testis, sperm and seminal plasma. Based on sialic acid content, the average concentrations of gangliosides in bovine testis, sperm and seminal plasma were 1.6, 2.25 and 0.30 nmoles/mg protein. Seven chromatographically distinguishable gangliosides were found. The structures of five testicular gangliosides were shown to be N-acetylneuraminylgalactosylglucosyl ceramide (GM3)1 ; N-acetylgalactosaminyl-(N-acetylneuraminyl)-galactosyglucosyl ceramide (GM2); (N-acetylneuraminyl)2-galactosylglucosyl ceramide (GD3); galactosyl-N-acetylgalactosaminyl-(N-acetylneuraminyl)-galactosylglucosyl ceramide (GM1 ); and N-acetylneuraminyl-galactosyl-N-acetylgalactosaminyl-(N-acetylneuraminyl)-galactosylglucosyl ceramide (GD1a). The structure of the major ganglioside constituent of bovine sperm was shown to be identical to GM3. Saturated fatty acids of even carbon numbers from C14 to C24 were found in gangliosides. Appreciable amounts of n-uncosanoic (C21) and n-tricosanoic (C23) acids were also present. Testis tissue contained the galactosyl- and sialyltransferases for the synthesis of N-acetylneuraminyl-galactosyl-N-acetylgalactosaminyl-(N-acetylneuraminyl)-galactosylglucosyl ceramide (GD1a) starting from glucosyl ceramide.

Note:
ACKNOWLEDGMENTS This research was supported in part by grant HD-07013 from the National Institute of Child Health and Human Development. T. W. K. is supported by research career development award GM-70596 from the National Institute of General Medical Science, Purdue University AES Journal Paper No. 6679.

Submitted on April 5, 1977
Accepted on May 20, 1977




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