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Biology of Reproduction, Vol 17, 769-779, Copyright © 1977 by Society for the Study of Reproduction

Nonhistone Chromosomal Proteins of the Developing Rat Testis

NATHANIEL C. MILLS 1, and ANTHONY R. MEANS 1

1 Dept. of Cell Biology, Baylor College of Medicine, Houston, Texas


Chromatins were isolated from rat testes at 5 day intervals during development and solubilized in sodium dodecyl sulfate. Nonhistone chromosomal proteins were then separated by electrophoresis on SDS-polyacrylamide gels. This technique resolved the proteins into 40-50 distinct bands separated primarily on the basis of molecular weight. The chromosomal proteins derived from testes containing large portions of supporting (immature Sertoli) cells, spermatogonia and primary spermatocytes (5-20 days of age) contain many high molecular weight proteins. Chromatin preparations enriched in round spermatid chromatin (65 days of age) showed diminished amounts of high molecular weight proteins with the exception of two bands at 81,000 and 87,000 molecular weight which appeared to be specific for spermatids. Analysis of chromatin from Sertoli cell-enriched (SCE) testes, which are devoid of germinal cells, revealed that most of the nonhistones present are proteins of less than 80,000 molecular weight. Between 22 and 40 days of age, a large decrease in proteins at 123,000 and corresponding increase in proteins at 48,000 molecular weight was noted in SCE testis preparations. Thus, the metabolically active cells (supporting cells or immature Sertoli cells, spermatogonia and primary spermatocytes) have many high molecular weight nonhistone proteins, whereas less active spermatids contain predominantly smaller species. These data suggest that the high molecular weight proteins may represent enzymes or structural proteins needed for mitosis, meiosis and cell growth.

Note:
ACKNOWLEDGMENTS This work was supported by NIH research grant HD-07503 and the Baylor Center for Population Research and Studies in Reproductive Biology (HD-07495). Anthony R. Means is the recipient of a Research Career Development Award from the NIH.

Submitted on May 18, 1977
Accepted on July 18, 1977




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