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Biology of Reproduction, Vol 19, 895-901, Copyright © 1978 by Society for the Study of Reproduction
1 Departments of Anatomy and Physiology,
Ralph L. Smith Research Center,
University of Kansas Medical Center,
Kansas City, Kansas 66103 Follicular development was analyzed in hamsters injected s.c.with a superovulatory dose of
PMS (30 IU) at 0900 h on Day 1 of the cycle (day of ovulation). Concurrently, the animals were
injected ip with 200 µCi of [3H] thymidine in an attempt to label the developing follicles. Groups
of 4 animals each were killed on each day of the estrous cycle after PMS administration and
autoradiographs prepared from serially sectioned ovaries. The most dramatic changes occur within 48 h after PMS injection. As early as 4 h after PMS,
preantral follicles with 4-8 or more layers of granulosa cells (stages 2-4) are mobilized. Twenty
antral follicles (stage 6) per ovary develop on Day 2 in PMS treated hamsters at least 16 h before
the normal number of 9 appear in controls. In addition, on Day 2, 10 stage 5 follicles (with incipient antral cavity) are present in control and PMS treated animals; on Day 3, all stage 5 follicles in
the PMS treated group develop into antral follicles. Therefore, after PMS administration, a total of
30 follicles per ovary are available for ovulation. The origin of these follicles in PMS treated hamsters was determined. On Day 1 at 1000 h,
preantral follicles with 6-7 layers of granulosa cells (stage 3) and 8 or more layers of granulosa
cells (stage 4) contributed approximately 10 follicles per ovary. In cyclic hamsters, only one half of
these follicles ovulate and the others undergo atresia. However, there were no atretic stage 5 or 6
follicles in the PMS treated animals. The remaining 20 follicles per ovary were derived primarily
from stage 2 follicles (preantral follicles with 4-5 layers of granulosa cells). In summary, PMS treatment enhances follicular growth rate and the number of follicles reaching
maturity in the cyclic hamster. The increased number of ovulations was caused by: (1) decreased
atresia in the developing follicles and (2) recruitment of stage 2 follicles, i.e., the reserve follicles.
PMS also caused interstitial edema and hypertrophy of the ovarian stroma as well as premature
accumulation of liquor folliculi in stages 3 and 4 follicles.
Note:
ACKNOWLEDGMENTS
The research was supported by grants from NIH
(HD 00596 and The Ford Foundation). D. C. acknowledges the financial support of training grants from
NICHD (5T01 HD 0025) and from The Ford Foundation.
We thank the NIAMDD program for providing
the PMS.
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