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Biology of Reproduction, Vol 20, 390-398, Copyright © 1979 by Society for the Study of Reproduction

Binding of Prostaglandin F2agr to Membranes of Shell Gland Muscle of Laying Hens: Correlations with Contractile Activity

MIKLOS TOTH 1, DAVID M. OLSON 1, , and FRANK HERTELENDY 1

1 Departments of Physiology and Medicine, St. Louis University School of Medicine, St. Louis, Missouri 63104


Specific binding of prostaglandin F2agr (PGF2agr) to a membrane fraction prepared from the shell gland muscle of laying hens was characterized as a concentration dependent, irreversible (at 0°C or 20°C) or partially reversible (at 37°C) interaction with a limited number of binding sites (about 1 pmol/mg protein). The 9 and 11 hydroxyl groups, the 5 (cis) double bond and the agr steric position of the heptanoic acid side chain of the PGF2agr molecule appear to be essential prerequisites for optimal binding. Properties of this binding reaction were compared to the characteristics of in vitro contractile responses of shell gland strips to PGF2agr. The results indicate that 1) both specific binding and contractile responses can be elicited with physiological concentrations of PGF2agr, 2) the binding of PGF2agr and the contractile response of the shell gland muscle are both very rapid processes which attain a maximum value within 1 min, 3) both the saturation of the membranes and the maximal contractile response are achieved at 2 x 10-6 M PGF2agr. However, half maximal binding can be demonstrated at 4 x 10-7 M, whereas half maximal response at 2 x 10-8 M PGF2agr, 4) both the binding affinity to PGF2agr binding sites and the contraction stimulating activity of PGE1 is substantially lower than those of PGF2agr.

Note:
ACKNOWLEDGMENTS We are indebted to Dr. H. V. Biellier of the University of Missouri, Columbia, for providing the experimental birds and to Dr. J. Pike, The Upjohn Company for donating the reference prostaglandins.

Submitted on June 20, 1978
Accepted on August 14, 1978




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Copyright © 1979 by the Society for the Study of Reproduction.