Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 20, 545-551, Copyright © 1979 by Society for the Study of Reproduction

The Metabolism and Nuclear Migration of Estrogen in Porcine Uterus throughout the Implantation Process

BEVERLEY A. PACK 1, C. L. BROOKS 2, W. R. DUKELOW 2, , and S. C. BROOKS 1

1 Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201
2 The Endocrine Research Unit, Michigan State University, East Lansing, Michigan 48824


Gilts and sows were inseminated at estrus and sacrificed from 0 to 25 days later. Minced uterine samples were incubated for 2 h with 17beta-[6, 7-3H]-estradiol ([6, 73H]-E2), 3 x 10-9 M and Na235SO4 (siml x 10-4 M) and the labeled metabolic products were extracted and analyzed. At the same time, endometrial samples were taken for E2 nuclear receptor assays. It was found that sulfurylation, insignificant at the time of insemination (Day 0), rose to a plateau by Day 5 (average 49.2 ± 7.6%) and continued at that level for at least 25 days of pregnancy. During and after implantation, the proportions of estrone, estrone sulfate and E2-3-sulfate were essentially the same as those seen in the cycling animal at diestrus: i.e., 18.7 ± 7.2%, 32.6 ± 6.4% and 16.6 ± 9.5%, respectively.

The E2 nuclear receptor level averaged 0.32 pmoles/mg DNA from the day of insemination to Day 8 of pregnancy then dropped to 0 where it remained until Day 17. However, instead of increasing to 1.00-1.50 pmoles/mg DNA as it would at the next estrus (Pack et al., 1978a), only a slight increase then occurred (average 0.09 pmoles/mg DNA), which was maintained at least until Day 25 of pregnancy.

Therefore, the combined conditions of a high level of estrogen sulfotransferase and E2 dehydrogenase activities with a low E2 nuclear receptor level are characteristic of implantation and early pregnancy in the pig.

Note:
ACKNOWLEDGMENTS This investigation was supported in part by U.S. Public Health Service Research Grant No. HD-8735 from the National Institute of Child Health and Human Development of the National Institutes of Health. The authors wish to thank Carl Christensen for his expert technical assistance and the entire staff of the Endocrine Research Unit at Michigan State University for their cooperation, assistance and advice throughout the course of these experiments.

Submitted on May 10, 1978
Accepted on September 1, 1978






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Copyright © 1979 by the Society for the Study of Reproduction.