Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 21, 39-46, Copyright © 1979 by Society for the Study of Reproduction

Changes in Uterine Protein Synthesis during Delayed Implantation in the Western Spotted Skunk and its Regulation by Hormones

RODNEY A. MEAD 1, ARTHUR W. ROURKE 1, , and ANN SWANNACK 1

1 Department of Biological Sciences, University of Idaho, Moscow, Idaho 83843


The uterine cornua of intact and castrate western spotted skunks were injected with 10 µCi of [3H]-leucine to study uterine protein synthesis during the 200-day preimplantation period and to determine the hormonal control of this process. The SDS gels of skunk plasma and uterine flushings contained 2 bands (MW 43,000 and 14,000) that were never observed in plasma samples. Estradiol and/or progesterone increased the quantity of the 14,000 MW component. The [3H]-leucine was incorporated into 2 protein fractions (fraction I with a MW of >200,000 and fraction II with a MW of 43,000) during the early and late delay periods. The amount of radioactivity incorporated into fraction II quadrupled between the early and late delay periods. There was a significant increase in incorporation of label into 3 protein fractions sim5 days prior to nidation, with fraction III (MW 24,000) being present for the first time. Uterine protein synthesis did not occur in castrate females, whereas progesterone significantly enhanced incorporation of precursor into fractions I, II and III, but did not replicate the condition observed during the early activation period. Estradiol alone or administered in conjunction with progesterone significantly inhibited synthesis of labeled proteins.

The preimplantation rise in progesterone appears to play an important role in enhancing the quality and quantity of uterine proteins, but the data suggest that other hormones may also be required to induce the complete pattern of protein synthesis observed just prior to nidation.

Note:
ACKNOWLEDGMENTS We would like to thank Richard Naskali for photographing the gels and Debbie Amos for assistance with the statistical analysis. This research was supported by the NICHHD grant no. HD 06556 and a grant from the University of Idaho Biomedical Research Council.

Submitted on December 19, 1978
Accepted on March 29, 1979




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