| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology of Reproduction, Vol 21, 9-18, Copyright © 1979 by Society for the Study of Reproduction
1 The Rockefeller University and Population Council,
The Rockfeller University,
New York, New York 10021 The process of mucification of the preovulatory cumulus oophorus in the rat was examined by
the scanning electron microscope (SEM). Induction of mucification in vitro by either LH or FSH
was also studied. Mucification in vivo was studied in follicular cumuli isolated at different times
during proestrus. The morphology of these cumuli was compared with that of postovulatory
cumuli isolated from the oviductal ampullae. For the in vitro studies, follicular cumuli were isolated
at early proestrus and incubated for various time periods in presence of ovine or rat preparations of
either LH or FSH. The beginning of mucus deposition in follicular cumuli was observed at about 5
h before the expected ovulation. Cumuli isolated 2 h before ovulation were totally embedded in
the extracellular matrix. In vitro, first stages of mucification were observed in cumuli incubated for
3 h in medium containing LH. Extracellular hyaluronidase-sensitive material completely covered
the cumuli by 20 h of culture in presence of either LH or FSH. In the absence of the gonadotropins,
mucification was not observed. Parietal granulosa tissue showed inability to mucify in the presence
of LH in vitro. No accumulation of extracellular material could be observed in cumuli cultured for
24 h in hormone free medium and then exposed to LH. It was concluded that the hyaluronidase
sensitive matrix is accumulated between the cumulus cells in the follicle before ovulation. This
intercellular mucus is secreted specifically by the cumulus cells as a direct response to gonadotropic
stimulation. The cultured cumulus cells lose their ability to respond to LH.
Accepted on February 9, 1979
This article has been cited by other articles:
|
|
 |
|
  N. Borg and M. Holland The effect of glycosaminoglycans on rat gametes in vitro and the associated signal pathway Reproduction, March 1, 2008; 135(3): 311 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Sutton-McDowall, M. Mitchell, P. Cetica, G. Dalvit, M. Pantaleon, M. Lane, R. B. Gilchrist, and J. G. Thompson Glucosamine Supplementation During In Vitro Maturation Inhibits Subsequent Embryo Development: Possible Role of the Hexosamine Pathway as a Regulator of Developmental Competence Biol Reprod, May 1, 2006; 74(5): 881 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shimada, M. Nishibori, Y. Yamashita, J. Ito, T. Mori, and J. S. Richards Down-Regulated Expression of A Disintegrin and Metalloproteinase with Thrombospondin-Like Repeats-1 by Progesterone Receptor Antagonist Is Associated with Impaired Expansion of Porcine Cumulus-Oocyte Complexes Endocrinology, October 1, 2004; 145(10): 4603 - 4614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kimura, Y. Konno, K. Miyoshi, H. Matsumoto, and E. Sato Expression of Hyaluronan Synthases and CD44 Messenger RNAs in Porcine Cumulus-Oocyte Complexes During In Vitro Maturation Biol Reprod, March 1, 2002; 66(3): 707 - 717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nagyová, B. C. Vanderhyden, and R. Procházka Secretion of Paracrine Factors Enabling Expansion of Cumulus Cells Is Developmentally Regulated in Pig Oocytes Biol Reprod, October 1, 2000; 63(4): 1149 - 1156. [Abstract] [Full Text]
|
|
|
|
|
|
C. Tempel, A. Gilead, and M. Neeman Hyaluronic Acid as an Anti-Angiogenic Shield in the Preovulatory Rat Follicle Biol Reprod, July 1, 2000; 63(1): 134 - 140. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
