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Biology of Reproduction, Vol 21, 301-308, Copyright © 1979 by Society for the Study of Reproduction

Hyaluronidase Does Not Disperse the Cumulus Oophorus Surrounding Bovine Ova

STEVEN P. LORTON 1, and NEAL L. FIRST 1

1 Endocrinology-Reproductive Physiology Program and Department of Meat and Animal Science, University of Wisconsin, Madison, Wisconsin 53706


Bovine follicular oocytes were collected and cultured in vitro with commercially prepared hyaluronidase or with in vitro capacitated bovine spermatozoa. These treatments did not result in the dispersion of the cumulus oophorus layers surrounding these ova. Preincubation of follicular ova in TC-199 with or without FSH, hCG, pronase, dithiothreitol, hyaluronidase or trypsin had no effect on the subsequent ability of hyaluronidase to disperse the cumulus oophorus. Ova collected from the oviducts within 2-3 h after ovulation were completely devoid of surrounding follicular cells in the absence of exposure to sperm hyaluronidase. Incubation of follicular ova in vitro in oviducts of estrous or pregnant heifers resulted in the removal of cumulus layers. The role of bovine sperm hyaluronidase in the fertilization process is discussed.

Note:
ACKNOWLEDGMENTS The authors would like to acknowledge the technical assistance of Ms. Berri Forman. This work was supported by the College of Agricultural and Life Sciences, University of Wisconsin, Madison; USDA-SEA Grant No. 12-14-5001-313 Mod. l; PHS Training Grant No. 5-T01-HD00104-10 from the NICHHD; and Grant No. 630-0505B from the Ford Foundation.

Submitted on March 11, 1979
Accepted on May 8, 1979






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Copyright © 1979 by the Society for the Study of Reproduction.