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Biology of Reproduction, Vol 21, 401-411, Copyright © 1979 by Society for the Study of Reproduction

Effects of Indomethacin on Ovulation and Luteal Function in Gilts

L. AINSWORTH 1, B. K. TSANG 2, B. R. DOWNEY 3, R. D. BAKER 3, G. J. MARCUS 1, , and D. T. ARMSTRONG 2

1 Animal Research Institute, Ottawa, Ontario, Canada K1A 0C6
2 Departments of Obstetrics and Gynecology and Physiology, University of Western Ontario, London, Ontario, Canada N6G 2J8
3 Department of Animal Science, Macdonald College of McGill University, Ste Anne de Bellevue, Quebec, Canada H0A 1C0


Experiments were carried out to determine the effects of inhibition of synthesis of prostaglandins by indomethacin on ovulation, oocyte maturation, luteinization and luteal function in prepubertal gilts treated with PMSG and hCG to induce follicular growth and ovulation. Indomethacin suppressed ovulation and the preovulatory rise in follicular fluid levels of prostaglandin E and prostaglandin F when administered at 20, 24 or 32 h after hCG injection. Oocyte maturation was not inhibited by indomethacin but progressive degeneration of the cumulus cell mass surrounding the oocyte occurred with time. Unruptured follicles in indomethacin treated animals progressively increased in size, blood and fibrin filled the central cavity and there was thickening and extreme vasodilatation of the thecal sheath; lutein cells lining the cavity appeared normal. Groups of PMSG/hCG treated gilts, treated with indomethacin 20 or 32 h after hCG treatment, had plasma progesterone profiles which were similar to those of untreated controls and indicated normal luteal function. These results support the hypothesis that ovarian synthesis of prostaglandin is a prerequisite in the sequence of events leading to follicle rupture and ovulation but do not implicate prostaglandins in oocyte maturation, or in development of normal luteal function.

Note:
ACKNOWLEDGMENTS These studies were supported in part by Quebec Agricultural Research Council Grant MCA-75-595 to R.D.B. and by Medical Research Council of Canada Grant MT 3392 and World Health Organization Grant 74228 to D.T.A. D.T.A. is an Associate of the Medical Research Council (Canada). The skillful technical assistance provided by G. Barbe, J. Lockhart, A. Peirce, J. Pika and A. Sutherland and the advice and surgical help provided by Dr. P. A. Martin is gratefully acknowledged. In addition, we thank Dr. W. D. Dorian, Merck, Sharpe and Dohme Canada Ltd., for the donation of indomethacin.

Submitted on January 3, 1979
Accepted on April 16, 1979




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Copyright © 1979 by the Society for the Study of Reproduction.