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Biology of Reproduction, Vol 21, 425-431, Copyright © 1979 by Society for the Study of Reproduction

Immunochemical Studies with Pregnant Mare Serum Gonadotropin

SUSAN WALKER FARMER 1, and HAROLD PAPKOFF 2

1 Hormone Research Laboratory, University of California, San Francisco, California 94143
2 Hormone Research Laboratory and Reproductive Endocrinology Center, University of California, San Francisco, California 94143


Immunological relationships among pregnant mare serum gonadotropin (PMSG), its subunits and equine pituitary gonadotropins (LH and FSH) were examined. For this purpose a homologous double antibody radioimmunoassay (RIA) for PMSG was developed and characterized. Levels of PMSG in serum samples collected from 2 mares during the first trimester of pregnancy were measured in the RIA and were found to be similar to values previously reported using bioassay and heterologous RIA techniques. Compared with PMSG which has been isolated from serum, preparations of this gonadotropin isolated from endometrial cup tissue were found to give parallel but slightly less potent (x0.3-0.9) inhibition curves. Extremely small amounts of PMSG were detected in pregnant mare urine. Isolated PMSG subunits were found to have a low degree of nonparallel cross reaction in the PMSG RIA; assay of heterologous subunit combinations revealed the importance of the beta-subunit for immunological recognition. Antisera to PMSG subunits were tested in additional RIAs. When PMSG-agr antiserum was used, all equine gonadotropins showed parallel inhibition curves, but these preparations demonstrated much less cross reaction with PMSG-beta antiserum. Equine pituitary gonadotropins showed significant cross reactions in the PMSG RIA while those of other species gave minimal and nonspecific cross reactions. These data, in conjunction with data obtained from RIAs employing antiserum to eLH and eFSH, demonstrate a very close immunological relationship between PMSG and eLH.

Note:
ACKNOWLEDGMENTS We thank Dr. Ted Hayashida for use of laboratory facilities, Drs. George Seidel and George Stabenfeldt for pregnant mare sera, Dr. Selna Kaplan for performing the iodination of PMSG, Mrs. Eleanor Rowley for helpful discussion of radioimmunoassay techniques, Mr. K. Steve LaForge for expert technical assistance and Dr. Choh Hao Li for critical reading of the manuscript. This work was supported in part by a grant from NIH (HD 05722).

Submitted on February 19, 1979
Accepted on April 16, 1979






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Copyright © 1979 by the Society for the Study of Reproduction.