Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HEWITT, K.
Right arrow Articles by GRINNELL, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HEWITT, K.
Right arrow Articles by GRINNELL, F.
Agricola
Right arrow Articles by HEWITT, K.
Right arrow Articles by GRINNELL, F.

Biology of Reproduction, Vol 21, 691-707, Copyright © 1979 by Society for the Study of Reproduction

Disappearance of Anionic Sites from the Surface of the Rat Endometrial Epithelium at the Time of Blastocyst Implantation

KATHRYN HEWITT 1, ALAN E. BEER 1, , and FREDERICK GRINNELL 1

1 Department of Cell Biology, The University of Texas Health Science Center at Dallas, Dallas, Texas 75235


Studies have been carried out to analyze possible changes in the distribution of anionic sites on the endometrial epithelium that might play a role in blastocyst implantation. Anionic sites were stained in nonfixed specimens at physiological pH and ionic strength utilizing polycationic ferritin and were subsequently observed by electron microscopy. Experiments were carried out using both in situ and in vitro labeling conditions, with either pregnant or pseudopregnant rats. There was a general reduction of anionic sites from Days 2-6 of pregnancy or pseudopregnancy until at Day 6 anionic sites were no longer detected. The results were similar on the mesometrial and antimesometrial sides of the uterus and on presumptive implantation sites (visualized by pontamine blue staining) and elsewhere on the endometrial epithelium. These findings suggest that the uterus undergoes a hormonally controlled loss of cell surface anionic sites, which may be related to the ability of the blastocyst to implant about Days 5-6 but not earlier.

Note:
ACKNOWLEDGMENTS We wish to thank Mr. Bill Sanders for his assistance. This research was supported by grants CA14609 and HD10413 from NIH.

Submitted on February 19, 1979
Accepted on May 23, 1979




This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
L. B. Schwartz, F. Naftolin, C. R. Lyttle, A. S. Penzias, E. L. Meaddough, and H. J. Kliman
Mouse Ascites Golgi (MAG) Mucin Expression and Regulation by Progesterone in the Rat Uterus
Reproductive Sciences, July 1, 2001; 8(4): 216 - 223.
[Abstract] [PDF]

Home page
 Biol. Reprod.Home page
J.K. Powell, S.R. Glasser, S. Woldesenbet, R.C. Burghardt, and G.R. Newton
Expression of Carbohydrate Antigens in the Goat Uterus During Early Pregnancy and on Steroid-Treated Polarized Uterine Epithelial Cells In Vitro
Biol Reprod, February 1, 2000; 62(2): 277 - 284.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1979 by the Society for the Study of Reproduction.