Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 21, 749-753, Copyright © 1979 by Society for the Study of Reproduction

Effect of Antivitellus Serum on Fertilization in the Mouse

Y. TSUNODA 1, and T. SUGIE 1

1 Department of Reproduction, National Institute of Animal Industry, Chiba-shi 280 Japan


Antisera against components of unfertilized mouse eggs other than zonae pellucidae were produced in 3 male rabbits and the effects of the antisera on fertilization were studied in the mouse. The antibody titers determined by using zona-free eggs in the cytotoxic test were 2-4 to 2-6. Treatment of eggs in vitro or passive immunization of female mice with antisera No. 1 and No. 3 did not inhibit fertilization, but treatment of intact eggs and passive immunization with antiserum No. 2 did inhibit fertilization. It was considered that the inhibition of fertilization after treatment of eggs or passive immunization of females with antiserum No. 2 was due to the contaminating antibody against the zona pellucida. The fertilizing capacity of spermatozoa was suppressed by treatment of sperm with all 3 antisera. The inhibitory effect of antiserum on fertilizing capacity of sperm disappeared, however, after absorption of antiserum with the ovary but not with liver or kidney. It is possible that spermatozoa and egg components other than the zona pellucida have a common antigen which induces an antibody which suppresses the fertilizing capacity of spermatozoa.

Note:
ACKNOWLEDGMENTS The authors are grateful to Drs. J. Mori, S. Shibuya and M. C. Chang for their advice and for reading the manuscript. Some of the results of the present study have appeared as an abstract in the Proceedings of the Symposium on Recent Advances in Reproduction and Regulation of Fertility, New Delhi, October 24-28th, 1978.

Submitted on March 15, 1979
Accepted on June 7, 1979






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Copyright © 1979 by the Society for the Study of Reproduction.