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Biology of Reproduction, Vol 21, 801-806, Copyright © 1979 by Society for the Study of Reproduction
1 Department of Zoology,
University of Washington,
Seattle, Washington 98195 To assess a possible role of the adenohypophysis in photorefractoriness, photosensitive and
photorefractory adult male white-crowned sparrows (Zonotrichia leucophrys) were treated with
single intravenous injections of 0, 0.5, 5, 20, 35, 50 or 500 ng of synthetic luteinizing hormone
releasing hormone (Syn-LHRH) in 0.9% saline solution. Serial samples of plasma were taken
immediately before and at 5, 20 and 60 min after injection. The plasma level of immunoreactive
luteinizing hormone (irLH) was not altered by the injection of saline solution alone, or by 0.5 or
5 ng Syn-LHRH in either photosensitive or photorefractory birds. Treatment with 20, 35, 50 or
500 ng Syn-LHRH, however, elicited significant and approximately equal increases in plasma levels
of irLH in both groups 5 min after injection. These responses are consistent with the hypothesis
that the site of photorefractoriness is at a level higher than the adenohypophysis.
Note:
ACKNOWLEDGMENTS
This investigation was supported by NSF Grant
No. PCM 77-17690 to D. S. Farner and NIH Grant No.
AM-05052 to J. W. Crim. We are indebted to Professor
B. K. Follett for the gift of anti-chicken-LH serum and
chicken-LH standards.
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